![]() The identification, in the adult, of cardiomyocyte turnover events and of cardiac progenitor cells (CPCs) has revolutionized the field of cardiovascular medicine. CITATION: Valente M, Araújo A, Esteves T, Laundos TL, Freire AG, Quelhas P, Pinto-do-Ó P* and Nascimento DS*. This is of interest to the general cardiovascular research community as it details methods for standardization and simplification of histo-morphometric evaluation of emergent heart therapies. Extending the use of this sampling to other types of in situ analysis is also illustrated through the assessment of neovas-cularization and cellular engraftment in a cell-based therapy setting. ![]() Protocols are provided for representative sampling of the ischemic mouse heart followed by morpho-metric analysis of the left ventricle. However, non-methodical and inadequate sampling of the left ventricle often leads to misinterpretations and variability, making direct study comparisons unreliable. Following functional evaluation, post-mortem histo-logical analysis is essential to assess morphological and molecular alterations underlying the effectiveness of treatment. Cardiac therapies are commonly tested preclinically in small-animal models of myocardial infarction.
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